Resumo O objetivo deste trabalho foi avaliar in vitro a citotoxicidade em fibroblastos do ligamento periodontal humano de três cimentos de retrobturação: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) e Super EBA(r). Uma cultura de fibroblastos primários do ligamento periodontal humano foi obtida anteriormente a fim de avaliar a citotoxicidade dos três extratos dos cimentos de retrobturação após 2 e 7 dias de endurecimento. As diluições em série destes extratos (1:1, 1:2, 1:4 e 1:8) foram avaliados em 1, 3 e 7 dias empregando o ensaio colorimétrico metil-tiazol-tetrazólio (MTT). A viabilidade celular foi calculada em base da porcentagem do grupo de controle negativo, que representou 100% de viabilidade de células. As análises estatísticas foram realizadas com o teste t, ANOVA e teste de Kruskal-Wallis a um nível de significância de 5%. Verificou-se que a principal diferença entre os cimentos de retrobturação estava nas diluições mais elevadas (p<0,05) e houve um comportamento semelhante nas diluições mais baixas (p>0,05). A viabilidade celular dos fibroblastos do ligamento periodontal humano foi superior para MTA Angelus(r) de 2 dias de endurecimento (p<0,05), em comparação com os outros materiais de retrobturação. Não houve diferença significante entre MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) de 7 dias de endurecimento. Super EBA(r) mostrou a menor percentagem da viabilidade celular nas diluições mais altas (p<0,05). Portanto, os cimentos de retrobturação MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) foram menos citotóxicos na diluição mais alta (1:1) em comparação com Super EBA(r).

Abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) and Super EBA(r). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(r) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(r) and EndoSequence Root Repair Material Putty(r). Super EBA(r) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(r) and EndoSequence Root Repair Material Putty(r) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(r).

Objectives: The purpose of the study was to assess “in vitro” bacterial penetration resistance using two different obturation techniques, vertical compaction and lateral compaction with two endodontic sealers, one based on polydimethylsiloxane and the other based on calcium hydroxide and epoxy resin against Enterococcus faecalis strain ATCC 29212. Methods: Eighty-four uniradicular teeth were randomly divided into 4 groups (n = 20) and two control groups (n = 4). Group I was obturated with lateral compaction technique and Sealer 26, group II with vertical compaction technique and Sealer 26, group III with lateral compaction technique and Roeko Seal and group IV with vertical compaction technique and Roeko Seal. Enterococcus faecalis was used as a bacterial marker and bacterial penetration was assessed monitoring the specimens every hour for the first 96 hours and after this every 12 hours over a period of 15 days. Results: Kruskal Wallis test (p = 0.696) was used to compare the four test groups and the Mann Whitney U test was used to compare pairs of groups. Nevertheless, no statistically significant differences were found comparing compaction techniques as endodontic sealers (Group I and II p=1.000, group III and IV p=0.296). No statistically significant differences were found when comparing endodontic sealers as compaction techniques (Group I and III p=0.328, group II and IV p=1.000). Although there was a maximum of 115 hours for group III and a minimum time of 18:21 hours for group I. Conclusions: No significant differences were found regarding bacterial penetration resistance between Roeko Seal and Sealer 26 in combination with lateral and vertical compaction techniques against a strain of Enterococcus faecalis.

Objetivo: Evaluar “in vitro” la resistencia a la penetración bacteriana utilizando dos diferentes técnicas de obturación, compactación vertical y lateral con dos selladores endodónticos, uno a base de polidimetilsiloxano y el otro a base de hidróxido de calcio y resina epóxica frente a una cepa de Enterococcus faecalis ATCC 29212. Materiales y Métodos: Ochenta y cuatro dientes uniradiculares se dividieron de manera aleatoria en 4 grupos (n=20) y dos grupos de control (n= 4). El grupo I se obturó con la técnica de compactación lateral y sellador Sealer 26, el grupo II se obturó con la técnica de compactación vertical y sellador Sealer 26, el grupo III se obturó con la técnica de compactación lateral y sellador Roeko Seal y el grupo IV se obturó con la técnica de compactación vertical y sellador Roeko Seal. Se utilizó Enterococcus faecalis como marcador bacteriano y la penetración bacteriana se evaluó cada hora durante las primeras 96 horas, posterior a esto cada 12 horas durante 15 días. Resultados: Se utilizó la prueba de Kruskal Wallis (p=0.696) para comparar los 4 grupos y la prueba de la U de Mann Whitney para comparar pares de grupos, al comparar las técnicas de compactación según el sellador endodóntico no se encontró diferencias estadísticamente significativas en ambos materiales (Grupo I y II p=1.000, Grupo III y IV p=0.296). Al comparar los selladores endodónticos según las técnicas de compactación no se encontró diferencias estadísticamente significativas (Grupo I y III p=0.328, Grupo II y IV p=1.00). Sin embargo se registró un tiempo máximo de 115 horas para el grupo III y un tiempo mínimo de 18:21 horas para el grupo I. Conclusiones: No se encontró diferencias estadísticamente significativas en cuanto a la resistencia a la penetración bacteriana entre los selladores Roeko Seal y Sealer 26 en combinación con las técnicas de compactación lateral y vertical frente a una cepa de Enterococcus faecalis.